| 胆碱脱氢酶与脂质体的重组 |
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| 引用本文： | 刘林,林其谁. 胆碱脱氢酶与脂质体的重组[J]. 中国生物化学与分子生物学报, 1990, 6(6): 527-532 |
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| 作者姓名： | 刘林 林其谁 |
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| 作者单位： | 中国科学院上海生物化学研究所 上海(刘林)，中国科学院上海生物化学研究所 上海(林其谁) |
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| 摘 要： | 用预先制备的脂质体与含Triton X-100的胆碱脱氢酶保温随后用Bio-beadsSM-2去除去垢剂而使酶重组到脂质体,Triton X-100的浓度,磷脂-蛋白比,Mg~(2+),pH等均影响重组,脂质体的大小在重组中不是关键因素,重组酶的活力比纯化酶增加70％,重组酶对PMS的Km,相转变温度及活化能均与线粒体内膜接近而不同于纯化酶。
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| 关 键 词： | 胆碱脱氢酶 脂质体 膜蛋白重组 膜结合酶 |
| 收稿时间： | 1990-12-20 |
Reconstitution of Choline Dehydrogenase and Liposome |
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| Liu,Lin Lin,Qi-shui. Reconstitution of Choline Dehydrogenase and Liposome[J]. Chinese Journal of Biochemistry and Molecular Biology, 1990, 6(6): 527-532 |
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| Authors: | Liu Lin Lin Qi-shui |
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| Affiliation: | (Shanghai Institute of Biochemistry, Academia Sinica, Shanghai |
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| Abstract: | Choline dehydrogenase has been incorporated into liposome through a method that Triton X-100 was directly removed from liposome-detergent-enzyme solutions by Bic-beads SM-2. Concentration of Triton X-100, lipid/protein ratio, Mg2+, pH etc had influence on the reconstitution. Size of liposome wasn't a key factor in reconstitution procedure. Activity of reconstituted enzyme was increased 70% of that of purifed enzyme. Km for PMS, transition temperature and activation energy in reconstituted enzymes were similar to that in mitochondrial inner membrane,but were different from that in purifed enzyme. |
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| Keywords: | Choline Dehydrogenase Liposome Reconstitution of Membrane protein Membrane-Bound Enzyme |
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