Expression of an Antigen Associated with Basal Bodies of Human Ciliated Epithelial Cells |
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Authors: | Mary T. Comer Mike Shires Nigel P. Goode Henry J. Leese Ludwik K. Trejdosiewicz Jennifer Southgate |
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Affiliation: | (1) ICRF Cancer Medicine Research Unit, St James's University Hospital, Leeds, LS9 7TF, UK;(2) Renal Research Unit, St James's University Hospital, Leeds, LS9 7TF, UK;(3) Department of Biology, University of York, P.O. Box 373, York, YO1 5YW, UK |
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Abstract: | The process and regulation of ciliogenesis in human epithelia is little understood and many components of the cilium and associated structures have not been characterised. We have identified a monoclonal antibody, LhS28, which recognises a 44,000–45,000Mr protein specifically associated with human ciliated epithelial cells. Immunoperoxidase labelling of formalin-fixed paraffin wax-embedded human tissues showed that LhS28 was expressed in the sub-apical zone of ciliated epithelial cells of the Fallopian tube and upper respiratory tract, but not ciliated ependyma, non-ciliated epithelia or testis containing developing spermatozoa. Immunoelectron microscopy demonstrated that the antigen recognised by LhS28 was associated with the basal body structure of the cilium and specifically with the 9+0 microtubule arrays. LhS28 should be a useful tool in the identification of ciliated cells in pathological specimens and for investigating mechanisms of ciliogenesis. |
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