| 微波辅助提取龙葵中总生物碱的研究 |
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| 引用本文： | 刘覃,陈晓青,蒋新宇,吴升德.微波辅助提取龙葵中总生物碱的研究[J].天然产物研究与开发,2005,17(1):65-69. |
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| 作者姓名： | 刘覃 陈晓青 蒋新宇 吴升德 |
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| 作者单位： | 中南大学化学化工学院,长沙,410083 |
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| 摘 要： | 本文对龙葵中总生物碱(Tocal steroidal alkaloids,)的微波辅助提取(Microwave-assisted extraction，MAE)工艺、作用机理及对成分的结构影响进行了系统的研究。以酸性染料比色法为分析手段，设计正交试验得到了最佳提取工艺：以95％乙醇为溶剂，料液比1：20，在585w下提取8min；用扫描电镜(SEM)对样品处理前后形貌进行了观察，揭示了微波作用机理与细胞结构变化有关。同时薄层色谱(TLC)、反相高效液相色谱(RP-HPLC)定性分析显示，微波对有效成分的结构没有造成影响。
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| 关 键 词： | 总生物碱 微波辅助提取 酸性染料比色法 95％乙醇 最佳提取工艺 龙葵 RP-HPLC 作用机理 细胞结构 有效成分 |
| 修稿时间： | 2004年7月23日 |
Study on the Microwave-Assisted Extraction of the Total Steroidal Alkaloids from Solanum nigrum L. |
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| LIU Qin,CHEN Xiao-qing,JIANG Xin-yu,WU Sheng-de.Study on the Microwave-Assisted Extraction of the Total Steroidal Alkaloids from Solanum nigrum L.[J].Natural Product Research and Development,2005,17(1):65-69. |
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| Authors: | LIU Qin CHEN Xiao-qing JIANG Xin-yu WU Sheng-de |
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| Institution: | LIU Qin,CHEN Xiao-qing*,JIANG Xin-yu,WU Sheng-de |
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| Abstract: | The microwave-assisted extraction(MAE) of the total steroidal alkaloids(TSA) from Solanum nigrum L.was systematically studied,including the optimum condition,mechanism of the extraction and the effect on the active components.The extract was analysed by acidic dye colorimetry and the optimized procedure determined by the orthogonal experiments are as follows:extraction with the 95% ethanol for 8 min under the power of 585 W.The Scanning Electron Microscopy(SEM) micrographs revealed that the difference of the extraction was related to the structural changes of the plant cells.Meanwhile,the results of the TLC and RP-HPLC showed MAE has no effect on the structures of the TSA. |
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| Keywords: | Solanum nigrum L total steroidal alkaloids microwave-assisted extraction |
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