Exploiting a natural auxotrophy for genetic selection |
| |
| Authors: | Ramage Elizabeth Gallagher Larry Manoil Colin |
| |
| Affiliation: | Department of Genome Sciences, University of Washington, Seattle, Washington, USA. |
| |
| Abstract: | We exploited the natural histidine auxotrophy of Francisella species to develop hisD (encodes histidinol dehydrogenase) as a positive selection marker. A shuttle plasmid (pBR103) carrying Escherichia coli hisD and designed for cloning of PCR fragments replicated in both attenuated and highly virulent Francisella strains. During this work, we formulated a simplified defined growth medium for Francisella novicida. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
