| 乙酸渗漏型丙酮酸高产菌的选育 |
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| 引用本文： | 刘立明,李寅,堵国成,钱崎峰,陈坚.乙酸渗漏型丙酮酸高产菌的选育[J].工业微生物,2002,32(3):10-14. |
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| 作者姓名： | 刘立明 李寅 堵国成 钱崎峰 陈坚 |
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| 作者单位： | 江南大学教育部工业生物技术重点实验室,无锡,214036 |
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| 基金项目： | 江苏省“九五”工业重大科技攻关项目(BG98015—3) |
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| 摘 要： | 对Torulopsis glabrata WSH-IP303进行NTG诱变，挑选以乙酸为补充碳源的平板上透明圈较大的菌落，经初筛和复筛，发现T.glabrataWSH-LQ307生产丙酮酸能力强且稳定。以乙酸为补充碳源摇瓶培养48h，其丙酮酸产量（46.2g/L)比出发菌株（38.3g/L)提高21%，采用该菌株在5L发酵罐上进行4批发酵实验，丙酮酸产量在64h最高可达68.7g/L，对葡萄糖的转化率为0.651g/g。
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| 关 键 词： | 光滑球拟酵母 丙酮酸发酵 丙酮酸脱羧酶 乙酸渗漏型 丙酮酸高产菌 选育 |
Breeding of high-pyruvate-producing Torulopsis glabrata with acetate as supplement carbon source |
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| LIU Li-ming,LI Yin,DU Guo-cheng,QIAN Qi-feng,CHEN Jian.Breeding of high-pyruvate-producing Torulopsis glabrata with acetate as supplement carbon source[J].Industrial Microbiology,2002,32(3):10-14. |
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| Authors: | LIU Li-ming LI Yin DU Guo-cheng QIAN Qi-feng CHEN Jian |
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| Abstract: | Mutants from T. glabrata WSH - IP303 which exhibited large transparent rings on the plate with acetic acid as an energy source, were obtained after NTG treatment . Among them, a mutant WSH - LQ307. Not only produced pyruvate to a high level with acetic acid as an energy source, but also showed strong genetic stability during the generation. In a defined medium containing glucose, acetic acid, NI-L,C1, salts and vitamins, the pyruvate production by T. glabrata WSH- LQ307 reached 46. 2 g/L for 48h of flask culture, which was 21% higher than the parent strain. Pyruvate concentration and yield reached 68.7 g/L and 0.651 g/g in 5 litre fermentor after 64h, respectively. |
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| Keywords: | Torulopsis glabrata pyruvate fermentation acetate-leaky auxotrophic mutants pyruvate decarboxylase (PDC) |
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