Coxsackievirus B3 entry into the host cell interferes with G-protein-mediated transmembrane signalling |
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Authors: | Jiri Novotny Petr Kvapil Jeronimo Cello Lennart A Ransnäs |
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Institution: | (1) Wallenberg Laboratory for Cardiovascular Research and Department of Clinical Virology, Gothenburg University, S-413 45 Gothenburg, Sweden |
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Abstract: | In the present work we used various cell lines in order to study the possible effect of coxsackievirus B3 (CVB3) entry on the adenylyl cyclase transmembrane signalling system. A significant decrease (by about 10–20%) was found in forskolin-augmented as well as in AlF
4
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- and GTP S-sensitive adenylyl cyclase activity in plasma membranes isolated from HeLa, HEp-2, Vero and green monkey kidney cells shortly (up to 60 min) preincubated with CVB3 (5 PFU/cell). Moreover, the ability of G-proteins derived from plasma membranes of infected cells to reconstitute AC activity in the cyc– mutant of S49 cells was also reduced. Content of G-protein subunits, however, remained unchanged after CVB3 attachment. Functional alterations in the G-protein-mediated adenylyl cyclase signalling system were accompanied by a marked decrease (by about 20–40%) of intracellular cAMP levels in virus-affected cells. These findings demonstrate clearly that CVB3 may affect functioning of the G-protein regulated adenylyl cyclase transmembrane signalling system in virus-sensitive cells as early as during the first period of its contact with the cellular plasma membrane. |
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Keywords: | adenylyl cyclase coxsackievirus B3 G-protein transmembrane signalling |
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