New Resources for Marine Genomics: Bacterial Artificial Chromosome Libraries for the Eastern and Pacific Oysters (Crassostrea virginica and C. gigas) |
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| Authors: | Charles Cunningham Jun-ichi Hikima Matthew J Jenny Robert W Chapman Guang-Chen Fang Chris Saski Mats L Lundqvist Rod A Wing Pauline M Cupit Paul S Gross Greg W Warr Jeff P Tomkins |
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| Institution: | (1) Marine Biomedicine and Environmental Sciences Center and Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Hollings Marine Laboratory, Charleston, SC 29412, USA;(2) Marine Resources Research Institute, South Carolina Department of Natural Resources, Charleston, SC 29412, USA;(3) Clemson University Genomics Institute, Clemson, SC 29634, USA;(4) Arizona Genomics Institute, 303 Forbes Hall, Tucson, AZ 85721, USA;(5) Department of Biology, University of New Mexico, 216 Castetter Hall, Albuquerque, NM 87131, USA;(6) Department of Biology, Woods Hole Oceanographic Institution, Woods Hole, MA 02543-1050, USA;(7) Present address: Department of Biology, University of New Mexico, NM, 87131, USA |
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| Abstract: | Large-insert genomic bacterial artificial chromosome (BAC) libraries of two culturally and economically important oyster species,
Crassostrea virginica and C. gigas, have been developed as part of an international effort to develop tools and reagents that will advance our ability to conduct
genetic and genomic research. A total of 73,728 C. gigas clones with an average insert size of 152 kb were picked and arrayed representing an 11.8-fold genome coverage. A total of
55,296 clones with an average insert size of 150 kb were picked and arrayed for C. virginica, also representing an 11.8-fold genome coverage. The C. gigas and C. virginica libraries were screened with probes derived from selected oyster genes using high-density BAC colony filter arrays. The probes
identified 4 to 25 clones per gene for C. virginica and 5 to 50 clones per gene for C. gigas. We conducted a preliminary analysis of genetic polymorphism represented in the C. gigas library. The results suggest that the degree of divergence among similar sequences is highly variable and concentrated in
intronic regions. Evidence supporting allelic polymorphism is reported for two genes and allelic and/or locus specific polymorphism
for several others. Classical inheritance studies are needed to confirm the nature of these polymorphisms. The oyster BAC
libraries are publicly available to the research community on a cost-recovery basis at |
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| Keywords: | BAC library crassostrea gigas crassostrea virginica oysters |
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