Immunochemical analysis of the structure of eukaryotic ribosomes |
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Authors: | Georg Stöffler Nora Fischer Karl-Heinz Rak Renate Ehrlich and Ira G Wool |
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Institution: | (1) Abteilung Wittmann, Max-Planck-Institut für Molekulare Genetik, Ihnestr. 63-73, D-1000 Berlin;(2) Department of Biochemistry, University of Chicago, 920 East 58th Street, 60637 Chicago, Illinois, USA |
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Abstract: | Summary Antibodies were prepared in rabbits and sheep to rat liver ribosomes, ribosomal subunits, and to mixtures of proteins from the particles. The antisera were characterized by quantitative immunoprecipitation, by passive hemagglutination, by immunodiffusion on Ouchterlony plates, and by immunoelectrophoresis. While all the antisera contained antibodies specific for ribosomal proteins, none had precipitating antibodies against ribosomal RNA. Rat liver ribosomal proteins were more immunogenic in sheep than rabbits, and the large ribosomal subunit and its proteins were more immunogenic than those of the 40S subparticle. Antisera specific for one or the other ribosomal subunit could be prepared; thus it is unlikely that there are antigenic determinants common to the proteins of the two subunits. When ribosomes, ribosomal subunits, or mixtures of proteins were used as antigens the sera contained antibodies directed against a large number of the ribosomal proteins.Abbreviations TP
total proteins—used to designate mixtures of proteins from ribosomal particles, hence TP80 is a mixtures of all the proteins from 80S ribosomes
- TP60
the proteins from 60S subunits
- TP40
the proteins from 40S particles |
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