The epitope structure in lipopolysaccharide recognized by the serotype 2-specific monoclonal antibody of Antinobacillus pleuropneumoniae |
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Authors: | Toyotsugu Nakai Kazuyoshi Kawahara Hirofumi Danbara Katsumi Kume |
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Institution: | Research Center for Veterinary Science of the Kitasato Institute, Kashiwa, Chiba, Japan;Department of Bacteriology, The Kitasato Institute, Minato-ku, Tokyo, Japan |
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Abstract: | Abstract: The polysaccharide structure recognized by a monoclonal antibody specific to serotype 2 lipopolysaccharide of Actinobacillus pleuropneumoniae was investigated using an enzyme-linked immunosorbent assay inhibition test. Lipopolysaccharide obtained from serotype 2, strain SH-15, was hydrolysed with acetic acid to liberate the polysaccharide portion, and the polysaccharide mixture was fractionated by gel filtration. The longer polysaccharide, composed of O -antigenic polysaccharide and core, fully inhibited the binding of monoclonal antibodies to a whole cell antigen of strain SH-15, whereas the core oligosaccharide without O -polysaccharide did not. No inhibition was observed with the monosaccharides which were the components of serotype 2 LPS. Enzyme-linked immunosorbent assay inhibition ability of O -polysaccharide was completely lost only by O -deacetylation. These results demonstrate that the epitope of the serotype-specific monoclonal antibody resided in O -polysaccharide of LPS and that the O -acetyl group was essential for the epitope structure. |
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Keywords: | Actinobacillus pleuropneumoniae Monoclonal antibody Serotype-specific O-polysaccharide |
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