Demonstration of D-xylose reductase and D-xylitol dehydrogenase in Pachysolen tannophilus |
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| Authors: | Karl L Smiley Paul L Bolen |
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| Institution: | (1) U.S. Department of Agriculture Agricultural Research Service Northern Regional Research Center, 1815 North University Street, 61604 Peoria, Illinois, USA |
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| Abstract: | Summary The yeast, Pachysolen tannophilus, can utilize the pentose D-xylose with accumulation of significant quantities of ethanol. Cell extracts of the organism contain NADPH-linked D-xylose reductase (aldose reductase EC 1.1.1.21) and NAD-dependent D-xylitol dehydrogenase (D-xylulose reductase EC 1.1.1.9). D-Xylose was required for induction of both the D-xylitol dehydrogenase and the D-xylose reductase. Neither enzyme was found in glucose grown cell-free extracts. |
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