B Cell Epitopes within VP1 of Type O Foot-and-mouth Disease Virus for Detection of Viral Antibodies |
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| Authors: | Shan-dian GAO Jun-zheng DU Hui-yun CHANG Guo-zheng CONG Jun-jun SHAO Tong LIN Shuai SONG Qing-ge XIE |
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| Affiliation: | Shan-dian GAO,Jun-zheng DU,Hui-yun CHANG **,Guo-zheng CONG,Jun-jun SHAO,Tong LIN,Shuai SONG , Qing-ge XIE (National Foot-,-mouth Disease Reference Laboratory,Key Laboratory of Animal Virology of Ministry of Agriculture,State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730046,China) |
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| Abstract: | In this study,the coding region of type O FMDV capsid protein VP1 and a series of codon optimized DNA sequences coding for VP1 amino acid residues 141-160(epitope1),tandem repeat 200-213(epitope2(+2)) and the combination of two epitopes(epitope1-2)was genetically cloned into the prokaryotic expression vector pPROExHTb and pGEX4T-1,respectively.VP1 and the fused epitopes GST-E1,GST-E2(+2)and GST-E1-2 were successfully solubly expressed in the cytoplasm of Escherichia coli and Western blot analysis demonstrat... |
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| Keywords: | Foot-and-mouth disease virus(FMDV) Serology Epitope ELISA |
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