Development of SNP Panels as a New Tool to Assess the Genetic Diversity,Population Structure,and Parentage Analysis of the Eastern Oyster (<Emphasis Type="Italic">Crassostrea virginica</Emphasis>) |
| |
| Authors: | Wilawan Thongda Honggang Zhao Dongdong Zhang Lauren N Jescovitch Ming Liu Ximing Guo Meagan Schrandt Sean P Powers Eric Peatman |
| |
| Institution: | 1.School of Fisheries, Aquaculture and Aquatic Sciences,Auburn University,Auburn,USA;2.Institute of Marine and Coastal Sciences and The New Jersey Agricultural Experiment Station, Haskin Shellfish Research Laboratory,Rutgers University,Port Norris,USA;3.Department of Marine Sciences,University of South Alabama,Mobile,USA;4.Center for Ecosystem Based Fisheries Management,Dauphin Island Sea Lab,Dauphin Island,USA |
| |
| Abstract: | Culture of the eastern oyster (Crassostrea virginica) is rapidly expanding. Combined with their continuing role as an environmental sentinel species and ecological model, this trend necessitates improved molecular tools for breeding and selection, as well as population assessment and genetic conservation. Here, we describe the development and validation of two panels of 58 single nucleotide polymorphism markers (SNPs) for the species. Population analyses revealed three distinct populations, based on FST values and STRUCTURE, among wild oysters sampled from Delaware Bay (1), northwest Florida (2), Alabama (2), Louisiana (2), and the Texas Gulf Coast (3), consistent with previous microsatellite and mtDNA analyses. In addition, utilizing the developed panels for parentage assignment in cultured oysters (Rutgers, New Jersey) resulted in a highly accurate identification of parent pairs (99.37%). The SNP markers could, furthermore, clearly discriminate between hatchery stocks and wild-sourced individuals. The developed SNP panels may serve as an important tool for more rapid and affordable genetic analyses in eastern oyster. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
