Cloning and characterization of G+C-rich, highly repeated DNA sequences from Porphyra yezoensis (laver), Rhodophyta |
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Authors: | Yuzuru Mizukami Hitoshi Kito Masahiko Kunimoto Masahiro Kobayashi |
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Institution: | (1) National Fisheries University, Shimonoseki, Yamaguchi, 759-6595, Japan;(2) Ishigaki Tropical Station, Seikai National Fisheries Research Institute, Ishigaki, Okinawa, 907-0451, Japan |
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Abstract: | G+C-rich sequences in the genomic DNA of Porphyrayezoensis (laver) were cloned and characterized. Sequence analyses of the genomic DNA inserted in fiveclones showed that the DNA contained long G+C-richstretches of more than 200 bp. These stretchesconsisted of more than 80% G+C residues. TheG+C-rich sequences were highly repeated andinterspersed throughout the genome of P.yezoensis and constituted about 6.0–6.6% of thegenome. Parts of these sequences were tandem repeatedin arrays. Hybridization experiments showed thatthese highly repeated, interspersed G+C-rich sequenceswere present in other species of Porphyra, butnot in species of the genera Grateloupia and Gelidium, suggesting that these sequences mightevolve rapidly among genomes, species and genera. |
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Keywords: | G+C-rich sequence interspersed DNA Porphyra yezoensis tandem repeated DNA |
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