Chlamydia psittaci ewe abortion agent: complete nucleotide sequence of the major outer membrane protein gene |
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Authors: | M.A. Pickett J.S. Everson I.N. Clarke |
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Affiliation: | Department of Microbiology, University of Southampton Medical School, Southampton General Hospital, Southampton, U.K. |
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Abstract: | The complete nucleotide sequence encoding the major outer membrane protein (MOMP) of Chlamydia psittaci strain A22/M, responsible for enzootic abortion of ewes (EAE), has been determined. An 800bp Eco RI/ Xba I fragment containing a portion of the MOMP coding sequence from C. trachomatis serovar L1 was used to probe a λL47.1 genomic library constructed from DNA obtained from C. psittaci EAE A22/M. The recombinant L47.1/EA1 was selected and contained the entire C. psittaci MOMP gene within a 7.5 kb Bam HI fragment. The DNA sequence revealed an open reading frame encoding 402 amino acids, including a 22 amino acid signal peptide, which exhibited 17/22 conservation with the signal peptide of C. trachomatis MOMP. The calculated molecular mass of the C. psittaci MOMP was 43 kDa. A comparison of the MOMP genes of C. psittaci and C. trachomatis revealed only 34% nucleotide sequence homology, but 65% amino acid homology. |
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Keywords: | Chlamydia psittaci Outer membrane protein Nucleotide sequence Ewe abortion agent |
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