Identification of an immediate-early salicylic acid-inducible tobacco gene and characterization of induction by other compounds |
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Authors: | Diana M Horvath Nam-Hai Chua |
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Institution: | (1) Laboratory of Plant Molecular Biology, The Rockefeller University, 1230 York Avenue, 10021 New York, NY, USA |
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Abstract: | Tobacco genes that are induced in response to salicylic acid (SA) treatment with immediate-early kinetics were identified by differential mRNA display. Detailed analysis of IS10a, one cDNA clone identified by this method, revealed induction within 30 min of treatment, with a peak of expression at 3 h, that decayed rapidly thereafter. Treatment with the protein synthesis inhibitor, cycloheximide (CHX), also caused induction of IS10a mRNA to comparable levels, but the IS10a mRNA continued to accumulate after 3 h of induction. In combination, CHX and SA led to a superinduction of IS10a mRNA levels that was also sustained. Half-maximal induction was evident at ca. 100–150 M SA. In addition to SA, induction of IS10a occurred to varying degrees upon treatment with acetylsalicylic acid, benzoic acid, 2,4-dichlorophenoxyacetic acid, methyl jasmonate, and hydrogen peroxide, whereas treatment with other compounds had no effect. The proteins encoded by IS10a and a second highly homologous cDNA show sequence similarity to UDP-glucose: flavonoid glucosyltransferases.Abbreviations 2,4-D
2,4-dichlorophenoxyacetic acid
- 4HBA
4-hydroxybenzoic acid
- aa
amino acid
- bp
base pair
- nt
nucleotide
- ASA
acetyl salicylic acid
- BA
benzoic acid
- CHX
Cycloheximide
- MJ
methyl jasmonate
- PCR
polymerase chain reaction
- SA
salicylic acid
- TMV
tobacco mosaic virus |
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Keywords: | auxin cycloheximide differential display glucosyltransferase hydrogen peroxide methyl jasmonate |
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