Inhibition of in vitro pre-mRNA splicing in S. cerevisiae by branched oligonucleotides |
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| Authors: | Carriero S Braich R S Hudson R H Anglin D Friesen J D Damha M J |
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| Affiliation: | Department of Chemistry, McGill University, Montreal, QC, Canada, H3A 2K6. |
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| Abstract: | A series of V- and Y-shaped nucleic acids, related to the splicing intermediates derived from S. cerevisiae actin pre-mRNA, were prepared. The effects of such branched nucleic acids (bNAs) on the efficiency of in vitro pre-mRNA splicing in yeast were studied. The exogenous bNAs each effect the efficiency of splicing, yet to different degrees, depending on the sugar composition and topology of the molecules. Y-shaped RNAs inhibited the formation of mRNA (i.e. RNA splicing) to the greatest extent. |
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