A new facile method for spin-labeling of oligonucleotides |
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| Authors: | Strube T Schiemann O MacMillan F Prisner T Engels J W |
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| Institution: | Institut für Organische Chemie, Johann Wolfgang Goethe-Universit?t, Marie Curie Strasse 11, D-60439 Frankfurt am Main, Germany. |
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| Abstract: | A new facile method for spin-labeling suitable for DNA and RNA oligonucleotides is presented. The nitroxide 3-ethenyl-2,2,5,5-tetramethyl-pyrrolin-1-yloxy was directly introduced during automated solid-phase synthesis by a Pd(0) cross coupling reaction. The main advantages of this procedure are the small amount of spin-label needed for the derivatisation of the oligonucleotide and the high coupling efficiency on the solid phase. |
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