Cyclohexene nucleic acids (CeNA) form stable duplexes with RNA and induce RNase H activity |
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| Authors: | Wang J Verbeure B Luyten I Froeyen M Hendrix C Rosemeyer H Seela F Van Aerschot A Herdewijn P |
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| Affiliation: | Laboratory for Medicinal Chemistry, Rega Institute, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B-3000 Leuven, Belgium. |
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| Abstract: | Cyclohexene nucleic acids (CeNA) were synthesized using classical phosporamidite chemistry. Incorporation of a cyclohexene nucleo-side in a DNA chain leads to an increase in stability of the DNA/RNA duplex. CeNA is stable against degradation in serum. A CeNA/RNA hybrid is able to activate E. Coli RNase H. resulting in cleavage of the RNA strand. |
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