Optimization of culturing conditions of <Emphasis Type="Italic">Porphyridium cruentum</Emphasis> using uniform design |
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Authors: | Juan Wang Bilian Chen Xiaozhen Rao Jian Huang Min Li |
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Institution: | (1) Department of Biotechnology, College of Life Sciences, Fujian Normal University, Fuzhou, 350108, China |
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Abstract: | The red microalga Porphyridium contains many valuable compounds such as polysaccharides, polyunsaturated fatty acids, and phycoerythrin (PE). In this study,
a uniform design method and regression analysis were used to investigate the effects of initial pH, light intensity, inoculation
ratio, and liquid volume in flask on the optimal biomass, exopolysaccharides (EPS), and PE production of Porphyridium cruentum in a batch culture at laboratory scale. Using regression analysis, we obtained the models to clarify the effects of individual
factors and their interactions on the biomass, EPS, and PE production of P. cruentum. The optimal condition for the biomass was the following: pH 5.0, light intensity 7098.0 lx, inoculation ratio 1:17.2, and
liquid volume 100.0 ml; for EPS was pH 5.0, light intensity 4501.0 lx, inoculation ratio 1:20, and liquid volume 100.3 ml;
while pH 8.0, light intensity 7100.0 lx, inoculation ratio 1:20, and liquid volume 100.3 ml was the best for PE production.
The maximum biomass 3.27 g/l, EPS production 543.1 mg/l, and PE production 132.0 mg/l were demonstrated by confirmatory experiment
to the optimum culture conditions in a reciprocal shaker. The statistical methods used in the present study are useful strategies
for optimizing of culture conditions for other microalgae. |
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Keywords: | Biomass Culture condition Exopolysaccharide Optimization Phycoerythrin Porphyridium cruentum Uniform design |
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