胶原性关节炎大鼠炎性细胞因子的动态观察

目的通过对胶原性关节炎(CIA)大鼠多个细胞因子的动态观察,了解其变化规律,为阐明RA发病机制及CIA大鼠的模型研究提供实验依据。方法建立CIA大鼠模型。分别于初次免疫后第0、10、20、30、40、50、60天记录大鼠的体重,测量大鼠后足体积和足垫厚度,计算关节炎指数;HE染色光镜下观察关节滑膜病理变化;采用ELISA方法检测大鼠血清中TNF-α、IL-1β和IL-6水平变化。结果造模成功后的CIA大鼠14 d左右出现炎症反应,30 d左右达高峰,以后炎症逐渐减轻。与同期空白对照组大鼠相比,CIA大鼠血清中TNF-α水平10 d明显升高(P<0.01),IL-1β和IL-6水平20 d明显升高(P<0.05),三者水平均30 d达高峰(P<0.01),以后持续存在,但水平有所降低。结论 CIA大鼠血清中TNF-α、IL-1β和IL-6在RA的整个病程中一直发挥着重要作用。

Dynamic observation of inflammatory cytokines in the rat models of collagen-induced arthritis

Objective To observe the changes of inflammatory cytokines in rat models of collagen-induced arthritis （CIA）. Methods One hundred and forty healthy rats （ male：female = 1 ： 1, body weight 170 ± 10 g） were used in this study. The CIA model was established by immunization with injection of type II bovine collagen into rats. The body weight, volume and thickness of hind paws, and arthritic indexes were measured. The pathological changes of arthral synovial tissues were examined by HE staining and light microscopy. The levels of serum TNF-α, IL-1β and IL-6 were detected by enzyme-linked immunosorbent assay （ELISA） on the day 10, 20, 30, 40, 50 and 60 after the first immunization. Resuits The inflammatory reaction approximately appeared on the 14th day, reached a peak on the 30th day, and then gradually decreased following the time. The concentration of serum TNF-α was increased on the 10th day （P 〈0.01 ）, and the concentrations of IL-1β and IL-6 were increased on the 20th day after the first immunization （ P 〈 0. 05 ）. The peak concentrations of TNF-α, IL-1β and IL-6 were all on the 30th day （ P 〈 0.01 ）, and then gradually decreased following the time. Conclusion TNF-α, IL-1β an IL-6 play important roles during the whole course of the rheumatoid arthritis.