主动脉缩窄术模型小鼠成模早期评价技术

目的利用超声多普勒技术分析建立主动脉缩窄小鼠模型早期评价方法。方法 C57BL/6J小鼠经开胸手术结扎主动脉弓,获得理论直径为0.4 mm的无名动脉远端主动脉弓重建血管;超声多普勒分析重建小鼠主动脉弓及其分支内血流流速,分析小鼠主动脉重建情况;记录小鼠生存情况,Spss16.0软件Kaplan-Meier curves分析小鼠存活率;将术后1周多普勒血流分析数据与术后4周左室壁厚度做相关性分析;生物信息学总结分析国内外立该模型的技术与评价,综合分析建立主动脉缩窄小鼠模型的超声多普勒早期评价方法,为相关研究者提供参考。结果术后8周时重建组(TAC)小鼠存活率为85.0%(n=40),而假手术组(Sham)小鼠存活率为96.4%(n=28);小鼠术后1周超声多普勒检测无名动脉血流/左颈总动脉血流比值(IA/LCCA)大于5.9时,术后4周舒张期左室后壁厚度(LVPWD)显著增厚占比达100%;IA/LCCA于5.9～10.7之间时与LVPWD成线性相关,当IA/LCCA大于10.7时线性关系下降,LVPWD增大趋势变缓。结论 TAC小鼠术后1周时使用多普勒血流分析,IA/LCCA比值大于5.9时,术后4周小鼠LVPWD即发生显著增厚,超声多普勒血流分析可以作为模型是否成功的早期评价方法。

Analysis of an early stage evaluation technique of mouse models of transverse aortic constriction

Objective To establish an evaluation technique with Doppler analysis on transverse aortic constriction （TAC） mouse model. Methods A 0. 4 mm reconstructed aortic was established theoretically, and the blood flow in the aor- ta and branches were analysed by Doppler analysis. The mortality of TAC mice was calculated and analyzed using SPSS 16.0 software. The correlation between Doppler analysis of the blood flow one week after reconstruction and echocardiography anal- ysis of the left ventricle （ LV ） wall thickness 4 weeks after reconstruction was analyzed. This technique was evaluated accord- ing to the references used at home and abroad. Results The survival rate was 85% （n =40） 8 weeks after reconstriction, while it was 96.4% in the sham group （n = 28 ）. The LV posterior wall at end-diastole （LVPWD） showed significance when the IA/LCCA flow ratio 〉 5.9. The IA/LCCA flow ratio had a liner correlation with the LVPWD when it was between 5.9 and 10. 7, and the liner correlation was decreased when the ratio was 〉 10. 7. Conclusions When the IA/LCCA flow ratio 〉 5.9 at one week after reconstruction, the LVPWD gives significant thickness 4 weeks after reconstruction and Doppler anal-ysis can be used as an early stage evaluation technique of mouse models of transverse aortic constriction.