鲫鱼汤对阿霉素肾病大鼠Th17细胞相关炎性因子表达的影响

目的研究鲫鱼汤对阿霉素肾病大鼠IL-17、IL-23、IL-1β和CXCR2等表达的影响,探讨其治疗作用和机制。方法成年Wistar雄性大鼠40只,随机分为鲫鱼汤组、厄贝沙坦组、肾病组、空白对照组,除空白对照组,其他三组给予尾静脉注射6.2 mg/kg体重阿霉素建立肾病模型,空白组注射等容量生理盐水。模型成功后,给予鲫鱼汤灌胃干预9周,每周检测12 h尿蛋白定量,检测终末血生化指标变化,光镜下观察大鼠肾脏病理改变,免疫组化检测IL-17、IL-23、IL-1β和CXCR2在肾脏的表达,ELISA法检测IL-17、IL-23和IL-1β在血液中的含量。结果鲫鱼汤组大鼠血白蛋白水平高于肾病组(P=0.03)低于空白组(P=0.01);鲫鱼汤组大鼠肾组织IL-17、IL-23、CX-CR2较肾病组表达减少(P=0.01,P=0.01,P=0.02),较空白组表达增多(P=0.04,P=0.007,P=0.05)。结论 Th17细胞相关炎性因子增多是阿霉素肾病大鼠的发病机制之一,鲫鱼汤可通过升高血白蛋白,降低IL-17、IL-23、CXCR2表达而发挥肾脏保护作用。

Positive effect of Crucian carp decoction on the expression of Thl7 cells inflammatory factors in rats with nephrosis induced by adriamycin

Objective To observe the therapeutic effect of Crucian carp decoction on rats with nephrosis in- duced by adriamycin, and explore its mechanism of action. Methods Fourty healthy 8-week old male Wistar rats were randomly divided into crucian carp decoction group, Irbesartan group, nephrosis group, and control group. The rat model of nephrosis was induced by a single tail vein injection of adriamycin 6.2 mg/kg body weight. The rats of the crucian carp decoction group were given oral gavage of crucian carp decoction once everyday for 9 weeks, and urinary protein was checked each week. All rats were killed at week 9. Histological changes were observed by light microscopy and blood bio- chemical indices were detected. IL-17, IL-23, IL-113, and CXCR2 expressions in the kidney were assessed by immunohis- tochemistry, Serum IL-17, IL-23, IL-113 levels were assayed by ELISA. Result Rats in the crucian carp decoction group had a higher level of albumin compared with that of the nephrosis group （ P = 0.03 ） , significantly lower than that of the control group（P = 0.01 ） and the expressions of IL-17, IL-23, and CXCR2 in the kidney were significantly lower than those in the nephrosis group （P = 0. 01,P = 0.01 ,P = 0. 02）, significantly higher than those of the control group （P = 0.04,P = 0. 007,P = 0.05）. Conclusions The increase of Th17 ceil inflammatory factors may be one of the pathogenetie mechanisms in rats with adriamycin-induced nephrosis. Crucian carp decoction can improve the albumin level of rats with nephrosis, and alleviate renal impairment induced by IL-17, IL-23 and CXCR2.