小刺猴头菌发酵液小分子指纹图谱与多糖含量相关性研究

试验研究了小刺猴头菌发酵液小分子指纹图谱与多糖含量的相关性。在小刺猴头菌摇床培养发酵液整个发酵周期内取得的样品经石油醚、二氯甲烷和乙酸乙酯依次萃取,HPLC检测分析。选择二氯甲烷层的3种小分子物质含量为主要研究对象,通过SAS软件和SIMCA-P 11.5软件对其与发酵液多糖进行相关性分析。结果表明:发酵液二氯甲烷层的3,4,5号峰小分子与发酵液多糖在含量变化上呈极显著相关(P<0.01)。

Study on the Correlation of Small Molecule Fingerprints and Polysaccharide Content of Hericium caput-medusae Fermented Liquid

The Hericium caput-medusae decompressing bottle fermentation in the cultivation of three kinds of organic solvent extraction in small molecule fingerprints and its correlation with fermented liquid polysaccharide were studied. Samples obtained in the whole fermentation period from shake cultivation fermented liquid of Hericium caput-medusae were detected and analyzed with petroleum ether, methylene chloride and ethyl acetate extract in turn. The correlation of 3 kinds of small molecules of methylene chlo- ride layer with fermented liquid polysaccharide was analyzed through SAS software and SIMCA-P soft- ware. The results show that small molecules of peaks 3, 4 and 5 of the fermented liquid methylene chlo- ride layer are very significantly correlated with polysaccharide content of the fermented liquid.