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A protocol for high-throughput extraction of DNA from rice leaves |
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| Authors: | Xin Xu Shinji Kawasaki Tatsuhito Fujimura Chuntai Wang |
| Affiliation: | (1) Key Laboratory for Biotechnology of National Commission for Nationalities, College of Life Science, South-Central University for Nationalities, 430074 Wuhan, P.R., China;(2) Plant Physiology Department, National Institute of Agrobiological Sciences, Kannondai 2-1-2, 305-8602 Tsukuba, Ibaraki, Japan;(3) Laboratory of Plant Genetic Engineering, University of Tsukuba, Tennoudai 1-1-1, 305-8572 Tsukuba, Ibaraki, Japan |
| Abstract: | Current DNA isolation methods have limitations between speed and purity in high-throughput molecular genetic analysis such as gene mapping and marker-assisted selection programs. We have optimized a simple and rapid method for isolating high-quality genomic DNA from rice that significantly minimizes time and the use of laboratory materials. One person can process as many as 384 samples in 2 h. The isolated DNA is suitable for polymerase chain reaction-based techniques and is stable for no less than 6 mo of storage at 4°C. |
| Keywords: | DNA isolation map-based cloning PCR amplification rice |
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