Changes in the activities of carbon metabolizing enzymes with pod development in lentil (<Emphasis Type="Italic">Lens culinaris</Emphasis> L.) |
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Authors: | Jyoti?Chopra Narinder?Kaur Email author" target="_blank">Anil?K?GuptaEmail author |
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Institution: | (1) Department of Biochemistry, Punjab Agricultural University, 141004 Ludhiana, India |
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Abstract: | Activities of some key enzymes of carbon metabolism sucrose synthase, acid and alkaline invertase, phosphoenol pyruvate carboxylase,
malic enzyme and isocitrate dehydrogenase were investigated in relation to the carbohydrate status in lentil pods. Sucrose
remained the dominant soluble sugar in the pod wall and seed, with hexoses (glucose and fructose) present at significantly
lower levels. Sucrose synthase is the predominant sucrolytic enzyme in the developing seeds of lentil (Lens culinaris L.). Acid invertase was associated with pod elongation and showed little activity in seeds. Sucrose breakdown was dominated
by alkaline invertase during the development of podwall, while both the sucrose synthase and alkaline invertase were active
in the branch of inflorescence. A substantial increase of sucrolytic enzymes was observed at the time of maximum seed filling
stage (10–20 DAF) in lentil seed. The pattern of activity of sucrose synthase highly paralleled the phase of rapid seed filling
and therefore, can be correlated with seed sink strength. It seems likely that the fruiting structures of lentil utilize phosphoenol
pyruvate carboxylase for recapturing respired carbon dioxide. Higher activities of isocitrate dehydrogenase and malic enzyme
in the seed at the time of rapid seed filling could be effectively linked to the deposition of protein reserves. |
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Keywords: | Lens culinaris leguminosae lentil carbon metabolism sucrose metabolism sucrose synthase sucrose phosphate synthase invertase phosphoenol pyruvate carboxylase malic enzyme isocitrate dehydrogenase |
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