Solution structure of the recombinant human oncoprotein p13
MTCP1 |
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Authors: | Yin-Shan Yang Laurent Guignard André Padilla François Hoh Marie-Paule Strub Marc-Henri Stern Jean-Marc Lhoste Christian Roumestand |
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Institution: | (1) Centre de Biochimie Structurale, CNRS-UMR 9955, INSERM-U414, Université de Montpellier I, Faculté de Pharmacie, 15 Avenue Charles Flahault, F-34060 Montpellier Cedex 1, France;(2) Unité INSERM U462, Hôpital Saint-Louis, F-75475 Paris, France |
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Abstract: | The human oncoprotein p13
MTCP1
is coded by the MTCP1 gene, a gene involved in chromosomal translocations associated with T-cell prolymphocytic leukemia, a rare form of human leukemia with a mature T-cell phenotype. The primary sequence of p13
MTCP1
is highly and only homologous to that of p14
TCL1
, a product coded by the gene TCL1 which is also involved in T-cell prolymphocytic leukemia. These two proteins probably represent the first members of a new family of oncogenic proteins. We present the three-dimensional solution structure of the recombinant p13
MTCP1
determined by homonuclear proton two-dimensional NMR methods at 600 MHz. After proton resonance assignments, a total of 1253 distance restraints and 64 dihedral restraints were collected. The solution structure of p13
MTCP1
is presented as a set of 20 DYANA structures. The rmsd values with respect to the mean structure for the backbone and all heavy atoms for the conformer family are 1.07 ± 0.19 and 1.71 ± 0.17 Å, when the structured core of the protein (residues 11–103) is considered. The solution structure of p13
MTCP1
consists of an orthogonal -barrel, composed of eight antiparallel -strands which present an original arrangement. The two -pleated loops which emerge from this barrel might constitute the interaction surface with a potential molecular partner. |
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Keywords: | leukemia oncogenic protein protein structure translocations |
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