Genetic relationships among clinical and environmental isolates ofVibrio cholerae from Australia |
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Authors: | Patricia M Desmarchelier John L Reichelt |
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Institution: | (1) Commonwealth Institute of Health, University of Sydney, 2006, New South Wales, Australia;(2) Australian Collection of Marine Micro-organisms, School of Botany, University of New South Wales, 2033 Kensington, New South Wales, Australia |
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Abstract: | DNA-DNA homology among twenty-nine isolates having the phenotypic properties ofVibrio cholerae was studied using the S1 endonuclease method. Ten strains ofV. cholerae O1 isolated from patients and from the environment in Australia showed greater than 88% homology with the neotype strain ofV. cholerae NCTC 8021. Strains of the non-O1 serotype isolated from a variety of clinical and environmental sources also showed a high level of relatedness, including four luminescent strains and a reference strain of the biotypealbensis. A group of sucrose-negative strains showed a low level of homology (40 to 43%) withV. cholerae, but 75% and 82% homology within the group. |
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