ADP-ribosyl proteins formed by pertussis toxin are specifically cleaved by mercury ions |
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| Authors: | T Meyer R Koch W Fanick H Hilz |
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| Institution: | Institut für Physiologische Chemie, Universit?t Hamburg, Germany. |
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| Abstract: | Various types of ADP-ribosyl protein conjugates were synthesized and their chemical stability was compared with that of cysteine-linked ADP-ribosyl groups as formed by incubation of transducin or Gi/Go proteins with NAD and pertussis toxin. Treatment with 0.1 mM HgCl2 specifically cleaved the cysteine-linked conjugates. This may provide a tool for the quantitation of modified Gi/Go proteins as well as of other acceptors modified by ADP-ribose at cysteine residues in the presence of other ADP-ribosyl proteins. |
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