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Transposition studies of mini-Mu plasmids constructed from the chemically synthesized ends of bacteriophage Mu |
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| Authors: | Thomas A Patterson Donald L Court Ginette Dubuc J J Michniewicz J Goodchild Ahmad I Bukhari and Saran A Narang |
| Institution: | a Laboratory of Molecular Oncology, National Cancer Institute, Frederick, MD 21701-1013, U.S.A. Tel. (301)698-1582 b Division of Biological Sciences, National Research Council of Canada, Ottawa, Canada KlA OR6) Tel. (613)990-3247 |
| Abstract: | We describe below the chemical synthesis of the right and left ends of bacteriophage Mu and characterize the activity of these synthetic ends in mini-Mu transposition. Mini-Mu plasmids were constructed which carry the synthetic Mu ends together with the Mu A and B genes under control of the bacteriophage λ pL promoter. Derepression of pL leads to a high frequency of mini-Mu transposition (5.6 × 10?2) which is dependent on the presence of the Mu ends and the Mu A and B proteins. Five deletion mutants in the Mu ends were tested in the mini-Mu transposition system and their effects on transposition are described. |
| Keywords: | Bacteriophage Mu synthetic ends mini-Mu plasmids λ pL promoter transposition activity oligodeoxy-nucleotides cat gene deletion |
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