| 酶促反应制备壳寡糖条件的研究 |
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| 引用本文： | 孙玉英,张继泉,王淑军,刘万顺,韩宝芹.酶促反应制备壳寡糖条件的研究[J].中国生物工程杂志,2008,28(11):67-71. |
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| 作者姓名： | 孙玉英 张继泉 王淑军 刘万顺 韩宝芹 |
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| 作者单位： | 1. 淮海工学院海洋学院
2. 中国科学院海洋研究所
3. 中国海洋大学生命科学与技术学部 |
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| 基金项目： | 淮海工学院引进人才科研启动项目 |
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| 摘 要： | 通过膜分离法从Microbacterium sp.OU01的发酵液中分离到内切壳聚糖酶ChiN，并对其酶解制备壳寡糖条件进行了优化，获得的酶解产物数均分子量为1200。经薄层层析与HPLC分析，降解产物中不含单糖，初步说明降解产物为壳寡糖。酶解制备壳寡糖反应条件温和，操作简便，为实现壳寡糖产业化奠定了基础。
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| 关 键 词： | 壳聚糖酶 壳寡糖 酶促反应 |
| 收稿时间： | 2008-05-26 |
| 修稿时间： | 2008-09-01 |
Preparation of oligo-chitosan by enzyme-catalyzed reaction |
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| SUN Yu-ying,ZHANG Ji-quan,WANG Shu-jun,LIU Wan-shun,HAN Bao-qin.Preparation of oligo-chitosan by enzyme-catalyzed reaction[J].China Biotechnology,2008,28(11):67-71. |
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| Authors: | SUN Yu-ying ZHANG Ji-quan WANG Shu-jun LIU Wan-shun HAN Bao-qin |
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| Abstract: | Endo-type chitosanase (ChiN) was isolated from the chitosanase fermentation broth by membrane separation techniques and the conditions that oligo-chitosan was prepared by ChiN were also optimized. The number average molecular weight of prepared oligo-chitosan is about 1200. Analysis by the methods of TLC and HPLC, no monosaccharide exists in the enzymatic product. So we confirm that the major enzymatic products are oligo-chitosan. Oligo-chitosan can be produced rapidly and inexpensively by enzyme-catalyzed reaction which has laid a basis for the large-scare preparation of oligo-chitosan. |
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| Keywords: | chitosanase oligo-chitosan enzyme-catalyzed reaction |
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