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Laser-diffusion measurements on δ-endotoxin crystals from Bacillus thuringiensis var. kurstaki HD1 |
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| Authors: | DB Sattelle CA Haniff WE Thomas DJ Ellar |
| Affiliation: | 1. A.F.R.C. Unit of Insect Neurophysiology and Pharmacology, Department of Zoology, University of Cambridge, Downing Street, Cambridge, CB2 3EJ, USA;2. Deparment of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QW U.K. |
| Abstract: | Laser light scattering has been employed to investigate changes in the hydrodynamic properties of δ-endotoxin crystals of Bacillus thuringiensis var. kurstaki HD1. Crystals are polydisperse. Estimates of the mean hydrodynamic diameter agree with those obtained by light microscopy. Sodium dodecyl sulphate (SDS) at a final concentration of 1% results in swelling of the toxin crystals. Dithiothreitol-induced solubilization of the swollen crystals indicates the importance of disulphide bridges to the maintenance of the assembled crystal. |
| Keywords: | Laser light scattering δ -Endotoxin structure (B thuringiensis) |
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