Reassembly of anAcetabularia mediterranea cell from the nucleus,cytoplasm, and cell wall |
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| Authors: | E. K. Pressman I. M. Levin L. S. Sandakhchiev |
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| Affiliation: | (1) Present address: Siberian Division of the Academy of Sciences, Institute of Organic Chemistry, Novosibirsk, U.S.S.R.;(2) Present address: Siberian Division of the Academy of Sciences, Institute of Cytology and Genetics, Novosibirsk, U.S.S.R. |
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| Abstract: | Summary A. mediterranea cells capable of full morphogenesis were reassembled from nuclei, cytoplasm, and cell wall fraction.Reassembly was performed stepwise with the recombination of cytoplasm and cell walls and finally a nucleus was implanted.Reassembly of anucleate cells was carried out by means of retransplantation of their own cytoplasm or transplantation of cytoplasm from another cell. Combinations between cytoplasm and cell walls of dark or light maintained cells were prepared. The nuclei were always transferred from light maintained cells. |
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