Clostridium botulinum type C produces a novel ADP-ribosyltransferase distinct from botulinum C2 toxin |
| |
| Authors: | K Aktories U Weller G S Chhatwal |
| |
| Affiliation: | 1. Rudolf-Buchheim-Institut für Pharmakologie Justus-Liebig-Universität, Frankfurter Str. 107, D-6300 Gießen, FRG;2. Institutfür Bakteriologie und Immunologie der Justus-Liebig-Universität, Frankfurter Str. 107, D-6300 Gießen, FRG |
| |
| Abstract: | The culture medium of certain strains of Clostridium botulinum type C contains two separable ADP-ribosyltransferases. Besides the ADP-ribosylation of actin due to botulinum C2 I toxin, a second microbial enzyme causes the mono-ADP-ribosylation of a eukaryotic protein with a molecular mass of about 20 kDa found in platelets, neuroblastoma X glioma hybrid cells, S49 lymphoma cells, chick embryo fibroblasts and sperm. The eukaryotic substrate is inactivated by heating and trypsin treatment. In contrast, the novel ADP-ribosyltransferase, which can be separated by DEAE-Sephadex chromatography, is largely resistant in the short term to trypsin digestion. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
