In vitro propagation of pummelo (Citrus grandis L. osbeck) |
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Authors: | K P Paudyal N Haq |
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Institution: | (1) Environmental Research Group, Lanchester Building, University of Southampton, SO17 1BJ Southampton, UK |
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Abstract: | Summary Several experiments were carried out to develop protocols for the in vitro propagation of pummelo (Citrus grandis L. Osbeck) using shoot-tip explants from seedlings. Murashige and Skoog (MS) medium supplemented with various concentrations
of 6-benzylaminopurine (BA) and thidiazuron (TDZ), singly or in combination with α-naphthaleneacetic acid (NAA), was used
to determine the rate of shoot proliferation. The response of explants to all concentrations of TDZ was very poor. After 6
wk culture, the most adventitious shoots per explant (average 5.2) were obtained on medium supplemented with 1.8 μM BA. NAA with cytokinin in the medium did not improve the rate of shoot multiplication significantly. Addition of 5.8 μM gibberellic acid in shoot-proliferation medium during the second subculture improved shoot elongation significantly. Shoot
multiplication increased 3.5-fold in each successive subculture. NAA was superior to indolebutyric acid for in vitro root induction. Over 75% of the shoots developed roots when transferred to half-strength MS medium with 1.3, 2.7, or 5.4
μM NAA. |
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Keywords: | micropropagation root induction shoot tip |
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