| 家蝇气味结合蛋白基因cDNA片段的克隆与序列分析 |
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| 引用本文： | 朱彬彬,姜勇,牛长缨,周兴苗,雷朝亮. 家蝇气味结合蛋白基因cDNA片段的克隆与序列分析[J]. 昆虫学报, 2005, 48(5): 804-809 |
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| 作者姓名： | 朱彬彬 姜勇 牛长缨 周兴苗 雷朝亮 |
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| 作者单位： | 华中农业大学昆虫资源研究所 |
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| 基金项目： | 湖北省自然科学基金项目（2001ABBl28） |
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| 摘 要： | 根据同源性设计特异性引物，采用RT-PCR方法扩增出了家蝇Musca domestica 2种气味结合蛋白基因cDNA片段，大小分别为381 bp和353 bp，分别命名为MdomOBP1(GenBank登录号：AY730350)和MdomOBP2(GenBank登录号：AY730351)。测序分析结果表明，它们具有气味结合蛋白的标志性结构域。对这2个片段推导的氨基酸序列进行同源性分析，结果表明两者与已报道的双翅目昆虫的气味结合蛋白的同源性分别达57%～88% 和52%～91%。
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| 关 键 词： | 家蝇 触角 气味结合蛋白 基因 克隆 序列分析 |
| 文章编号： | 0454-6296(2005)05-0804-06 |
| 收稿时间： | 2004-09-14 |
| 修稿时间： | 2004-12-06 |
Cloning and analysis of cDNA fragments of two odorant binding proteins from the antenna of Musca domestica |
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| ZHU Bin-Bin,JIANG Yong,NIU Chang-Ying,ZHOU Xing-Miao,LEI Chao-Liang. Cloning and analysis of cDNA fragments of two odorant binding proteins from the antenna of Musca domestica[J]. Acta Entomologica Sinica, 2005, 48(5): 804-809 |
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| Authors: | ZHU Bin-Bin JIANG Yong NIU Chang-Ying ZHOU Xing-Miao LEI Chao-Liang |
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| Affiliation: | Huazhong Agricultural University |
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| Abstract: | Two cDNA fragments of two different odorant binding protein (OBP) genes in Musca domestica were amplified with two pairs of specific primers by RT-PCR, and the lengths of them were 381 bp named MdomOBP1 (GenBank accession no.: AY730350) and 353 bp named MdomOBP2 (GenBank accession no.: AY730351) respectively. Sequencing and analysis showed that these two OBP cDNA fragments were characterized by typical conservative Cys. Deduced amino acid sequences were highly similar to that of six OBPs from Diptera, with sequence identities of 57%-88% for MdomOBP1 and 52%-91% for MdomOBP2. |
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| Keywords: | Musca domestica antenna odorant binding protein gene cloning sequence analysis |
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