A rapid and sensitive liquid chromatographic assay for epoxide hydrase. |
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| Authors: | S Nesnow C Heidelberger |
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| Institution: | McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, Wisconsin 53706 USA |
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| Abstract: | A rapid method for the assay of epoxide hydrase activity is described. 3-Methylcholanthrene-11,12-oxide is employed as substrate and high speed liquid chromatography is used to separate and quantitate trans-11,12-dihydro-11,12-dihydroxy-3-methylcholanthrene (product) formation. The determination of product at picomole levels can be obtained. |
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