Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction |
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| Authors: | P Chomczynski N Sacchi |
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| Affiliation: | 1. Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA 19129;2. Department of Bioengineering, Temple University, Philadelphia, PA 19122 |
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| Abstract: | A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples. |
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