Analysis of Aspergillus niger transformants for single site integration and vector recombination |
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| Authors: | Georg Mohr Danuta Wilmanska Karl Esser |
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| Institution: | (1) Lehrstuhl für Allgemeine Botanik, Ruhr Universität Bochum, Postfach 10 21 48, D-4630 Bochum, Federal Republic of Germany;(2) Present address: Department of Molecular Genetics, The Ohio State University, 43210-1292 Columbus, Ohio, USA;(3) Present address: Institute of Microbiology, University of Lodz, Ul. Banacha 12-16, PL-90237 Lodz, Poland |
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| Abstract: | Summary In order to understand the mechanism of integrative transformation, bulk DNA from several transformants of Aspergillus niger was assaved for the presence of the heterologous vector p3SR2. In all cases vector sequences and recombinant vector fragments were found. Transformant T31-6 containing seven plasmid-probed bands was studied in detail. Recloning of six of these plasmids in Escherichia coli revealed that internal recombination events had produced deletions and insertions. Only one plasmid was isolated among 402 analysed clones that had involved A. niger DNA. Sequence analysis revealed that the integration of vector p3SR2 occurred via its A. nidulans amdS
+ sequence. These data support the assumption that the integration of multiple copies of p3SR2 occurred at a single genomic site.Offprint requests to: K. Esser |
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