Quantitation of DNA sequences in environmental PCR products by a multiplexed, bead-based method |
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| Authors: | Spiro Alexander Lowe Mary |
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| Institution: | Physics Department, Loyola College in Maryland, 4501 N. Charles Street, Baltimore, MD 21210, USA. |
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| Abstract: | A first application of a multiplexed, bead-based method is described for determining the abundances of target sequences in an environmental PCR product. Target sequences as little as 0.3% of the total amount of DNA can be quantified. Tests were conducted on 16S ribosomal DNA sequences from microorganisms collected from contaminated groundwater. |
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