Adoptive transfer of cells sensitized in vitro into mice in a skin allograft assay |
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Authors: | M M Kemeny P H Sugarbaker |
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Institution: | Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20205 USA |
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Abstract: | In these experiments we investigated the ability of adoptively transferred in vitro-sensitized cells to cause an accelerated rejection of skin allografts. The survival of B10.BR or B10.D2 skin grafts on B6AF1 mice was measured. It was determined that 5 × 107in vitro-sensitized cells were required for a consistent accelerated skin allograft rejection. Attempts to optimize sensitization using syngeneic mouse serum were unsuccessful. In vitro-sensitized lymphocytes were specific in their activity toward skin allografts, but were nonspecific in their lysis of tumor targets. Inadvertant transfer of alloantigen with in vitro-sensitized cells was not responsible for accelerated graft rejection. This work demonstrates that cells sensitized in vitro can cause specific accelerated skin allograft rejection in normal mice. |
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