Irrigation and Seed Quality Development in Rapid-cycling Brassica: Seed Germination and Longevity

Irrigation of rapid-cycling brassica (Brassica campestris [rapa]L.)plants either ended 16 or 24 days after pollination (DAP) orcontinued throughout the experiment (control). Seeds were harvestedserially from these plants during their development and maturation.The earlier irrigation to the mother plant ended, the earliermass maturity (end of seed-filling phase) occurred, the lowerthe final seed dry weight, and the more rapid the decline inseed moisture content. The onset of ability to germinate normallyoccurred as early as 12 DAP, when seeds were less than half-filled.The onset of ability to tolerate rapid enforced desiccation(to 10% moisture content) occurred at 16 DAP. Desiccation tolerancedeveloped within most seeds in both populations about 5 d soonerin seeds harvested from plants in which irrigation was stoppedat 16 DAP than in control plants, but maximum desiccation toleranceoccurred at about 28 DAP in all treatments. Survival curves(percentage normal germinationvs.period of storage) of seedshermetically stored at 40 °C with 15% moisture content conformedto negative cumulative normal distributions, and provided acommon estimate of the standard deviation of the frequency distributionof seed deaths in time for seed lots harvested at differenttimes from the three environments (