Design and use of conditional MHC class I ligands |
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Authors: | Toebes Mireille Coccoris Miriam Bins Adriaan Rodenko Boris Gomez Raquel Nieuwkoop Nella J van de Kasteele Willeke Rimmelzwaan Guus F Haanen John B A G Ovaa Huib Schumacher Ton N M |
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Affiliation: | Division of Immunology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands. |
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Abstract: | Major histocompatibility complex (MHC) class I molecules associate with a variety of peptide ligands during biosynthesis and present these ligands on the cell surface for recognition by cytotoxic T cells. We have designed conditional MHC ligands that form stable complexes with MHC molecules but degrade on command, by exposure to a defined photostimulus. 'Empty MHC molecules' generated in this manner can be loaded with arrays of peptide ligands to determine MHC binding properties and to monitor antigen-specific T-cell responses in a high-throughput manner. We document the value of this approach by identifying cytotoxic T-cell epitopes within the H5N1 influenza A/Vietnam/1194/04 genome. |
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