Tetanus Toxin Inhibition of K+ -Stimulated [3H]GABA Release from Developing Cell Cultures of the Rat Cerebellum |
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| Authors: | Brian R. Pearce Anthony L. Gard Gary R. Dutton |
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| Affiliation: | Department of Pharmacology, College of Medicine, University of Iowa, Iowa City, Iowa, U.S.A. |
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| Abstract: | Abstract: The effect of tetanus toxin pretreatment on K+ -stimulated [3H]γ-aminobutyric acid release from neuron-enriched cerebellar cell cultures at various stages during their development in vitro was assessed. Tetanus toxin had little inhibitory effect on immature (1-3-day-old) cultures, but markedly reduced K+-evoked [3H]γ-aminobutyric acid release from 7- and 14-day-old cultures (∼80% inhibition). It is suggested that cerebellar neurons in culture develop tetanus toxin-sensitive transmitter release mechanisms similar to their in vivo counterparts. |
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| Keywords: | Tetanus toxin [3H]γ-Aminobutyric acid release Cerebellar cell cultures |
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