Selective generation of leukotriene B4 by tracheal epithelial cells from dogs |
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| Authors: | M J Holtzman H Aizawa J A Nadel E J Goetzl |
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| Institution: | 1. Cardiovascular Research Institute, University of California, San Francisco, California 94143 USA;2. Howard Hughes Medical Institute, University of California, San Francisco, California 94143 USA;3. the Department of Medicine, University of California, San Francisco, California 94143 USA;4. the Department of Physiology, University of California, San Francisco, California 94143 USA |
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| Abstract: | The incubation of suspensions of canine tracheal epithelial cells of greater than 95% purity with arachidonic acid (25-200 micrograms/ml) for 60-120 min resulted in the generation of a maximum of 36.2 +/- 9.1 picomoles of leukotriene B4/10(6) cells, less than 2.0 picomoles of leukotrienes C4, D4, and E4/10(6) cells, and 1030 +/- 463, 767 +/- 500, and 324 +/- 100 picomoles/10(6) cells of 15-, 12-, and 5-hydroxy-eicosatetraenoic acids, respectively (mean +/- SEM, n = 8). The identity of leukotriene B4 was established by chromatographic and spectral properties, by reactivity with mono-specific anti-plasma, and by the chemotactic activity for neutrophils. Thus, the epithelium may be an important source of mediators of inflammation and hypersensitivity of pulmonary airways. |
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| Keywords: | LT leukotriene HETE hydroxy-eicosatetraenoic acid HPLC high-performance liquid chromatography |
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