The occurrence of polyadenylate sequences in bacteria and yeast |
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| Authors: | M Edmonds D W Kopp |
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| Affiliation: | 1. Department of Applied Life Science, Jeju National University, Jeju-do, 63243, Republic of Korea;2. [chanchanhee] Inc., Jeju, 63243, Republic of Korea;3. Gwanggyo Yonsei Skin Clinic, Gyung-gi do, 16507, Republic of Korea;4. Subtropical/tropical Organism Gene Bank, Jeju National University, Jeju, 63243, Republic of Korea;1. State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin, 300350, China;2. Collaborative Innovation Center for Biotherapy and School of Medicine, Nankai University, Tianjin, 300071, China;3. Department of Biochemistry and Molecular Biology, College of Life Sciences, Nankai University, Tianjin, 300071, China;4. Jiangxi Key Laboratory of Natural Products and Functional Foods, Jiangxi Agricultural University, Nanchang, 330045, China;1. College of Marine Science and Engineering, College of Life Science, Nanjing Normal University, Nanjing, Jiangsu, 210023, China;2. Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, Lianyungang, Jiangsu, 222005, China;1. Institute of Fisheries Science, National Taiwan University, 1 Roosevelt Road, Sec. 4, Taipei 106, Taiwan;2. Department of Nursing, Yuanpei University, Hsinchu City 300, Taiwan;3. Institute of Cellular and Organismic Biology, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei 115, Taiwan;4. Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica, 23-10 Dahuen Rd., Jiaushi, Ilan 262, Taiwan;1. College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, 266109, China;2. Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Life Sciences, Qingdao Agricultural University, Qingdao, 266109, China;3. Department of Animal Sciences, Auburn University, Auburn, AL, 36849, USA;1. Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai, China;2. Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China;3. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China;4. Laboratory of Biochemistry and Molecular Biology, Ningbo University, Ningbo, PR China;5. Key Laboratory of Conventional Freshwater Fish Breeding and Health Culture Technology Germplasm Resources, Suzhou Shenhang Eco-technology Development Limited Company, Suzhou, PR China |
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| Abstract: | The steady state level of AMP-rich polynucleotides has been measured in the total RNA of E. coli and S. cerevisiae by hybridization to polythymidylate covalently bonded to cellulose. In both cells, the levels of polyadenylate were an order of magnitude below the nearly one percent found in the total RNA of mammalian cells. The poly A from E. coli had an electrophoretic mobility similar to that from mammalian cells previously reported as 180–200 nucleotides in length. That from yeast was more hetereogenous with much of it migrating more rapidly than transfer RNA during electrophoresis. |
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