Growth-Promoting Action of Adenosine-Containing Dinucleotide on Neuroblastoma Cells: Detection of Adenosine-Cytidine Dinucleotide (ApCp) in Neurofibroma (NF1) Extracts |
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Authors: | Taeko Hotta Kiyofumi Asai Naohito Takeda Hideo Yoshizumi Akira Tatematsu Keiko Nakanishi Yaman Z Eksioglu Ichiro Isobe Taiji Kato |
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Institution: | Department of Bioregulation Research, Nagoya City University Medical School, Nagoya, Japan;Faculty of Pharmacy, Meijo University, Nagoya, Japan |
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Abstract: | Abstract: Neurofibroma type 1 tissue was investigated for the presence of growth-promoting activity on human neuroblastoma cells. The activity was isolated by gel filtration and reversed-phase column chromatographs from neurofibroma type 1 extracts. An adenosine-containing dinucleotide (adenylyl(3'-5')cytidine-3'-phosphate) was identified as one of the major components of the activities by its enzymatic fragmentation and liquid chromatography/mass spectrometry. Synthetic adenosine-containing dinucleotide derivatives such as cytidyl(3'-5')adenosine, cytidyl(2'-5')adenosine, adenylyl(3'-5')cytidine, and adenylyl(2'-5')cytidine showed a similar action. Cytidyl(3'-5')adenosine, cytidyl(2'-5')adenosine, and adenylyl(2'-5')cytidine, which are able to release a free adenosine through enzymatic hydrolysis, in particular elicited a strong activity corresponding to that of adenosine with the highest action. These results suggest that neuroblastoma cells are able to use adenosine-containing dinucleotides as well as mononucleotides for their survival and proliferation. |
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Keywords: | Neurofibroma Von Recklinghausen Neuroblastoma Dinucleotide Adenosine dinucleotide |
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