Isolation and characterization of mutated alcohol oxidases from the yeast Hansenula polymorpha with decreased affinity toward substrates and their use as selective elements of an amperometric biosensor |
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| Authors: | Kostyantyn V Dmytruk Oleh V Smutok Olena B Ryabova Galyna Z Gayda Volodymyr A Sibirny Wolfgang Schuhmann Mykhailo V Gonchar Andriy A Sibirny |
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| Affiliation: | 1.Institute of Cell Biology,NAS of Ukraine,Lviv,Ukraine;2.Department of Metabolic Engineering,Rzeszow University,Rzeszow,Poland;3.Anal. Chem.-Elektroanalytik and Sensorik,Ruhr-Universit?t Bochum,Bochum,Germany |
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| Abstract: | Background Accurate, rapid, and economic on-line analysis of ethanol is very desirable. However, available biosensors achieve saturation at very low ethanol concentrations and thus demand the time and labour consuming procedure of sample dilution. |
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