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Chitin-graft-poly(2-methyl-2-oxazoline) enhanced solubility and activity of catalase in organic solvent |
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| Authors: | Kensuke Naka Rie Yamashita Tohru Nakamura Akira Ohki Shigeru Maeda Keigo Aoi Akinori Takasu Masahiko Okada |
| Affiliation: | a Department of Applied Chemistry and Chemical Engineering, Faculty of Engineering, Kagoshima University, Korimoto, Kagoshima 890-0065, Japan b Department of Applied Biological Sciences, Faculty of Agricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan |
| Abstract: | Catalase from bovine liver was lyophilized from an aqueous solution containing chitin-graft-poly(2-methyl-2-oxazoline) (3), which was synthesized by the reaction of 52% deacetylated chitin (1) with living poly(2-methyl-2-oxazoline) (2). The rate of consumption of H2O2 in chloroform catalyzed by the lyophilized catalase with 3 was enhanced more than 10 times that by catalase without 3. The dispersibility and solubility of lyophilized catalase with 3 in chloroform were improved in comparison with catalase itself. |
| Keywords: | enzyme activity solubility catalase chitin derivative oxazoline derivative organic solvent chitin |
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