| 云南铁角蕨与泸山铁角蕨的关系:来自叶绿体rbcL、trnL-F和rps4-trnS序列的证据 |
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| 引用本文： | 李春香,陆树刚.云南铁角蕨与泸山铁角蕨的关系:来自叶绿体rbcL、trnL-F和rps4-trnS序列的证据[J].植物分类学报,2006,44(3):296-303. |
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| 作者姓名： | 李春香 陆树刚 |
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| 作者单位： | 1. 中国科学院南京地质古生物研究所现代古生物学和地层学国家重点实验室,南京,210008
2. 云南大学生态学与地植物学研究所,昆明,650091 |
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| 基金项目： | 中国科学院资助项目;中国科学院知识创新工程项目 |
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| 摘 要： | 云南铁角蕨Asplenium yunnanense与泸山铁角蕨Asplenium lushanense的分类是一个悬而未决的问题。本文对云南铁角蕨与泸山铁角蕨及近缘类群的叶绿体rbcL基因和trnL-F、rps4-trnS基因间隔区序列进行PCR扩增和序列分析；并与已经发表的铁角蕨属植物的相应序列进行比较，发现云南铁角蕨与泸山铁角蕨的rbcL基因和trnL-F、rps4-trnS基因间隔区序列之间均未表现出差异，因此叶绿体DNA序列的证据不能将云南铁角蕨与泸山铁角蕨分开，从而从分予系统学方而证明云南铁角蕨与泸山铁角蕨是一对亲缘关系非常近的物种。
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| 关 键 词： | 云南铁角蕨 泸山铁角蕨 叶绿体DNA序列 亲缘关系 |
| 收稿时间： | 08 23 2004 12:00AM |
| 修稿时间： | 04 22 2005 12:00AM |
Relationship of Asplenium yunnanense and A. lushanense inferred from the sequence analysis of chloroplast rbcL, trnL-F and rps4-trnS |
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| LI Chun-Xiang,LU Shu-Gang.Relationship of Asplenium yunnanense and A. lushanense inferred from the sequence analysis of chloroplast rbcL, trnL-F and rps4-trnS[J].Acta Phytotaxonomica Sinica,2006,44(3):296-303. |
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| Authors: | LI Chun-Xiang LU Shu-Gang |
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| Institution: | 1.State Key Laboratory of Palaeobiology and Stratigraphy of Nanjing Institute of Geology and Palaeontology, the Chinese Academy of Sciences, Nanjing 210008, China; 2 .Instttute of Ecology and Geobotany, Yunnan University, Kunming 650091, China |
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| Abstract: | The relationship between Asplenium yunnanense Franch. and Asplenium lushanense C. Chr. has long been in dispute, and here was evaluated based on the sequence analysis of chloroplast rbcL gene, trnL-F and rps4-trnS intergenic spacers. No sequence difference in the studied chloroplast DNA regions was found between Asplenium lushanense and A. yunnanense, but some nucleotide differences in the corresponding DNA fragments were found among other Asplenium species. So, A. yunnanense and A. lushanense cannot be distinguishable in terms of chloroplast DNA sequences, and the very close relationship between them is further affirmed based on the evidence from molecular data. |
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| Keywords: | Asplenium yunnanense Asplenium lushanense chloroplast DNA sequence relationship |
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