An Escherichia coli expression-based method for heme substitution |
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Authors: | Woodward Joshua J Martin Nathaniel I Marletta Michael A |
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Institution: | Department of Chemistry, Lawrence Berkeley National Laboratory, University of California, Berkeley, 211 Lewis Hall, Berkeley, California 94720, USA. |
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Abstract: | Heme reconstitution with porphyrin analogs is a powerful approach toward understanding the molecular function of heme proteins; present methods, however, have not proven to be generally useful. Here we describe the development and application of an expression-based method for introducing modified porphyrins. The approach allows efficient incorporation of heme analogs using a widely available bacterial strain and offers an attractive alternative to present reconstitution methods that subject proteins to harsh, denaturing conditions. |
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